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Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.

机译：钴离子螯合亲和色谱法纯化脑中性α-D-甘露糖苷酶并将其与酸性α-D-甘露糖苷酶分离。

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摘要

A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.

机译：描述了一种利用Co 2+螯合亲和色谱法纯化中性α-D-甘露糖苷酶并将其与猴脑酸性α-D-甘露糖苷酶分离的方法。选择性结合到金属离子螯合柱上的中性酶可通过pH 7.5的Tris洗脱，并且一步纯化得到80倍以上，回收率为100％。

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期刊名称 Biochemical Journal
作者
R Mathur; A S Balasubramanian;
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作者单位

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年(卷),期 1984(222),1
年度 1984
页码 261–264
总页数 4
原文格式 PDF
正文语种
中图分类分子生物学;
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机译：金属亲和色谱下可逆固定化螯合亲和性表面活性剂在反相吸附剂上的蛋白质和多肽分离
2. Purification of Human Serum Immunoglobulins Using Immobilized Metal Affinity Chromatography with Ethylenediamine Triacetic Acid as Chelating Agent [J] . Gonzalez-Ortega Omar, Guzman Roberto Journal of liquid chromatography and related technologies . 2015,第1a4期

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3. Immobilized iminodiacetic acid (IDA)-type Cu2+ -chelating membrane affinity chromatography for purification of bovine liver catalase. [J] . Yang L, Jia L, Zou H, Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences . 1999,第3期

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4. Separation and Purification of Ganoderic Acids by Macroporous Adsorption Resin and High Speed Countercurrent Chromatography [C] . ZHU ZHONGMIN, LI YE, HUAZHENGGEN, The 7th international medicinal mushroom conference . 2013

机译：大孔吸附树脂和高速逆流色谱法分离和纯化灵芝酸
5. Separation and purification of biomolecules by affinity chromatography based on cellulose and glass membranes. [D] . Guo, Wei. 2003

机译：通过基于纤维素和玻璃膜的亲和色谱法分离和纯化生物分子。
6. Purification and characterization of rat epididymal-fluid alpha-D-mannosidase: similarities to sperm plasma-membrane alpha-D-mannosidase. [O] . D R Tulsiani, M D Skudlarek, S K Nagdas, 1993

机译：大鼠附睾液α-D-甘露糖苷酶的纯化和表征：与精子血浆膜α-D-甘露糖苷酶相似。
7. Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein and Peptide Separations under Metal Affinity Chromatography [O] . Omar González-Ortega, Roberto Guzmán 2014

机译：金属亲和色谱下蛋白质和肽分离反相吸附剂上螯合亲和力表面活性剂的可逆固定

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase.

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