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From field courses to DNA barcoding data release for West Papua - making specimens and identifications from university courses more sustainable

机译:从西巴布亚的实地课程到DNA条形码数据发布-使大学课程中的标本和标识更具可持续性

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摘要

The diversity of insects collected during entomological field courses at the University of West Papua (UNIPA), Indonesia, is studied using DNA barcoding tools. The results were compared with public data available for West Papua in the Barcode of Life Data System. During two training courses in 2013 and 2015, 1,052 specimens of insects were collected at eight sites near Manokwari in northern West Papua. The DNA sequences obtained from these specimens were assigned to 311 Barcode Index Numbers (BINs) and represent species in 27 families of Lepidoptera, Hymenoptera and Coleoptera. Of those BINs, 294 (95%) were new to West Papua. The study suggests that DNA barcoding applied to university courses achieves several goals, including capacity building and hands-on experience in molecular biodiversity assessment. In addition, it can provide valuable biodiversity data that are globally available to researchers for further studies.
机译:使用DNA条形码工具研究了印度尼西亚西巴布亚大学(UNIPA)昆虫学野外课程中收集的昆虫多样性。将结果与生命条形码系统中可用于西巴布亚的公共数据进行了比较。在2013年和2015年的两次培训课程中,在西巴布亚北部Manokwari附近的八个地点收集了1,052个昆虫标本。从这些标本中获得的DNA序列被分配给311条码索引号(BINs),代表了鳞翅目,膜翅目和鞘翅目的27个科中的物种。在这些BIN中,有294(95%)个是西巴布亚的新用户。该研究表明,将DNA条形码应用于大学课程可以实现几个目标,包括能力建设和分子生物多样性评估的实践经验。此外,它还可以提供有价值的生物多样性数据,研究人员可以在全球范围内使用它们进行进一步研究。

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