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美国卫生研究院文献>Biomedical Optics Express
>Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection
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Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection
Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue.
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