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Off-axis digital holographic camera for quantitative phase microscopy

机译:用于定量相显微镜的离轴数字全息相机

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摘要

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM.
机译:我们提出并实验证明了一种数字全息照相机,可以将其安装到常规显微镜的照相机端口上,以便在短相干照明和单次拍摄中以自参考配置获得数字全息图。厚厚的全息光栅通过衍射对包含样本信息的光束进行二维过滤。经滤波的光束产生干涉仪的参考臂。与基于针孔的傅立叶滤波相比,基于厚光栅的高角度选择性的空间滤波方法降低了对角位移的对准灵敏度。薄的全息光栅的添加改变了厚光栅引入的相干平面倾斜,从而在整个视场上产生了高可见度的干涉。对获取的全场离轴全息图进行处理,以检索样本的幅度和相位信息。该系统产生与标准商业DHM提取的相位图像在质量上相似的颊部细胞相位图像。

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