Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers

摘要

BackgroundInsect cells can serve as host systems for the recombinant expression of eukaryotic proteins. Using this platform, the controlled expression of ¹⁵N/¹³C labelled proteins requires the analysis of incorporation paths and rates of isotope-labelled precursors present in the medium into amino acids. For this purpose, Spodoptera frugiperda cells were grown in a complex medium containing [U-¹³C6]glucose. In a second experiment, cultures of S. frugiperda were grown in the presence of ¹⁵N-phenylalanine.