The nucleotide addition cycle of RNA polymerase is controlled by two molecular hinges in the Bridge Helix domain

摘要

BackgroundCellular RNA polymerases (RNAPs) are complex molecular machines that combine catalysis with concerted conformational changes in the active center. Previous work showed that kinking of a hinge region near the C-terminus of the Bridge Helix (BH-HC) plays a critical role in controlling the catalytic rate.