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Preparation of an epitope-based recombinant diagnostic antigen specific to anti-phospholipase A2 receptor 1 antibodies

机译：抗磷脂酶A2受体1抗体特异的基于表位的重组诊断抗原的制备

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摘要

BackgroundAccording to recent studies, the phospholipase A2 receptor 1 (PLA2R1) may be used as a biomarker to diagnose idiopathic membranous nephropathy (iMN). Moreover, the immune-dominant regions of PLA2R1 have been identified. The aim of the present study was to construct a diagnostic antigen based on the immune-dominant region of PLA2R1 and develop a specific serological detection method for PLA2R1 antibodies.

机译：背景技术根据最近的研究，磷脂酶A2受体1（PLA2R1）可用作诊断特发性膜性肾病（iMN）的生物标记。而且，已经鉴定出PLA2R1的免疫优势区域。本研究的目的是基于PLA2R1的免疫优势区域构建诊断抗原，并开发针对PLA2R1抗体的特异性血清学检测方法。

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期刊名称 BMC Biotechnology
作者
Hua Li; Yonghua Xu; Weiming Yang; Xiaohong Huang;
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作者单位

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年(卷),期 2018(18),-1
年度 2018
页码 31
总页数 6
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词
Phospholipase A2 receptor 1 (PLA2R1) Prokaryotic expression Chromatographic purification ELISA;

机译：磷脂酶A2受体1（PLA2R1）;原核表达;色谱纯化;ELISA;

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专利

1. REVISITING ANTI-PHOSPHOLIPASE A2 RECEPTOR ANTIBODIES AND ITS ANTIGEN AS SPECIFIC BIOMARKERS IN IDIOPATHIC MEMBRANOUS NEPHROPATHY [J] . Lin L., Xu J., Pan X., Nephrology. . 2016,第Suppla2期

机译：特发性膜性肾病中抗磷脂酶A2受体抗体及其抗原作为特定生物标志物的研究
2. Revisiting Anti-phospholipase A2 Receptor Antibodies and Its Antigen as Specific Biomarkers in Idiopathic Membranous Nephropathy [J] . Li Lin, Jing Xu, Xiaoxia Pan, Hong Kong Journal of Nephrology . 2015,第2aSupplementa1期

机译：回顾性抗磷脂酶A2受体抗体及其抗原作为特发性膜性肾病的特异性生物标志物
3. Outcomes of primary membranous nephropathy based on serum anti-phospholipase A2 receptor antibodies and glomerular phospholipase A2 receptor antigen status: a retrospective cohort study [J] . Peihong Yin, Junxian Wang, Wenyi Liang, Renal failure. . 2020,第1期

机译：基于血清抗磷脂酶A2受体抗体和肾小球磷脂酶A2受体抗原状况的原发性膜肾病的结果：回顾性队列研究
4. Human Synthetic, Semi-Synthetic, and Na?ve Immune Libraries for Efficient Isolation of Antigen Specific Antibodies Isolation of Antigen Specific Antibodies [C] . Man-Seok Ju, Sang Taek Jung PEGS . 2016

机译：人合成，半合成和Na ve've免疫文库，用于有效地分离抗原特异性抗体的分离分离抗原特异性抗体
5. Distribution of circulating and glomerular IgG subclasses against full-length anti-phospholipase A2 receptor and its epitopes in primary membranous nephropathy [D] . Li, Lee Chuan 2015

机译：原发性膜性肾病中针对全长抗磷脂酶A2受体及其表位的循环和肾小球IgG亚类的分布
6. Outcomes of primary membranous nephropathy based on serum anti-phospholipase A2 receptor antibodies and glomerular phospholipase A2 receptor antigen status: a retrospective cohort study [O] . Peihong Yin, Junxian Wang, Wenyi Liang, 2020

机译：基于血清抗磷脂酶A2受体抗体和肾小球磷脂酶A2受体抗原状况的原发性膜肾病的结果：回顾性队列研究
7. Preparation of an epitope-based recombinant diagnostic antigen specific to anti-phospholipase A2 receptor 1 antibodies [O] . Hua Li, Yonghua Xu, Weiming Yang, 2018

机译：制备特异于抗磷脂酶A2受体1抗体的表位的重组诊断抗原
8. In vitro Antigen-Specific Antibody Response to the Species-Specific Surface Protein Antigens of Typhus Group Rickettsiae by Human Peripheral Blood Mononuclear Cells. Generation of an Antigen-Dependent Suppressor T Cell [R] . Misiti, J., Dasch, G. A. 1985

机译：体外抗原特异性抗体对人外周血单个核细胞对斑疹伤寒组立克次体的物种特异性表面蛋白抗原的反应。产生抗原依赖性抑制T细胞

Preparation of an epitope-based recombinant diagnostic antigen specific to anti-phospholipase A2 receptor 1 antibodies

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