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Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

机译：南美白对虾环糊精糖基转移酶在大肠杆菌中的异源表达及其在2-O-α-D-吡喃葡萄糖基-L-抗坏血酸生产中的应用

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BackgroundCyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical industries.

机译：背景环糊精葡糖基转移酶（CGTase）可以将L-抗坏血酸（L-AA，维生素C）转化为2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G），在食品，化妆品和药品中显示出广泛的应用行业。

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期刊名称 BMC Biotechnology
作者
Yujia Jiang; Jie Zhou; Ruofan Wu; Fengxue Xin; Wenming Zhang; Yan Fang; Jiangfeng Ma; Weiliang Dong; Min Jiang;
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作者单位

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年(卷),期 2018(18),-1
年度 2018
页码 53
总页数 10
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词
Cyclodextrin glucanotransferase Optimized codons Glycosyl donors 2-O-α-D-glucopyranosyl-L-ascorbic acid;

机译：环糊精葡糖基转移酶;优化密码子;糖基供体;2-O-α-D-吡喃葡萄糖基-L-抗坏血酸;

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专利

1. Coexpression of folding accessory proteins for production of active cyclodextrin glycosyltransferase of Bacillus macerans in recombinant Escherichia coli [J] . Kim SG, Kweon DH, Lee DH, Protein Expression and Purification . 2005,第2期

机译：在重组大肠杆菌中共表达折叠辅助蛋白以产生活性的Macerans芽孢杆菌活性环糊精糖基转移酶
2. Enzymatic transformation of 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) by immobilized α-cyclodextrin glucanotransferase from recombinant Escherichia coli [J] . Ziehen Zhang, Jianghua Li, Long Liu Journal of molecular catalysis, B. Enzymatic . 2011,第3a4期

机译：固定化重组大肠杆菌中的α-环糊精葡糖基转移酶对2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G）的酶促转化
3. Enzymatic Transformation of 2-O-α-D-glucopyranosyl-L-ascorbic Acid by a-cyclodextrin Glucanotransferase from Recombinant Escherichia coli [J] . Zichen Zhang, Jianghua Li, Long Liu, Biotechnology and bioprocess engineering . 2011,第1期

机译：重组大肠杆菌α-环糊精葡糖基转移酶对2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的酶促转化
4. Cloning, Overexpression and Immunological Detection/Purification of Recombinant B. Macerans; Cyclodextrin Glycosyltransferase in E.coli [C] . Bernard Y.Tao, Namsoo Han, K.C.P.Lee Asia-Pacific biochemical engineering conference;APBIOCHEC'97 . 1997

机译：重组Macerans的克隆，过表达和免疫学检测/纯化;大肠杆菌中的环糊精糖基转移酶
5. Identification of factors limiting heterologous lipase expression in the cytoplasm and the periplasm as well as display on cell surface of Escherichia coli. [D] . Xu, Yali. 2008

机译：鉴定限制异源脂肪酶在细胞质和周质中表达以及在大肠杆菌细胞表面上展示的因素。
6. Heterologous Expression of Cyclodextrin Glycosyltransferase my20 in Escherichia coli and Its Application in 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid Production [O] . Kai Song, Jingjing Sun, Wei Wang, 2021

机译：环糊精糖基转移酶Me20在大肠杆菌中的异源表达及其在2-O-α-D-吡喃葡萄糖基-L-抗坏血酸生产中的应用
7. Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous prpD and malK in Escherichia coli and Its Transglycosylation Application in Production of Rebaudioside [O] . Wenju Shu, Hongchen Zheng, Xiaoping Fu, 2020

机译：通过在大肠杆菌的内源性PRPD和MALL中的共同表达及其在莱鲍迪甙生产中的基础源性PRPD和MALC的糖基转移酶UGT76G1的异源生产。

Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production

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