BackgroundBasidiomycete high-redox potential laccases (HRPLs) working in human physiological fluids (pH 7.4, 150 mM NaCl) arise great interest in the engineering of 3D-nanobiodevices for biomedical uses. In two previous reports, we described the directed evolution of a HRPL from basidiomycete PM1 strain CECT 2971: i) to be expressed in an active, soluble and stable form in Saccharomyces cerevisiae, and ii) to be active in human blood. In spite of the fact that S. cerevisiae is suited for the directed evolution of HRPLs, the secretion levels obtained in this host are not high enough for further research and exploitation. Thus, the search for an alternative host to over-express the evolved laccases is mandatory.
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机译：背景技术在人类生理液（pH 7.4，150 mM NaCl）中工作的担子菌高氧化还原电位漆酶（HRPL）引起了人们对生物医学用途的3D纳米生物装置工程的兴趣。在先前的两个报告中，我们描述了来自担子菌PM1菌株CECT 2971的HRPL的定向进化：i）在酿酒酵母中以活性，可溶和稳定的形式表达，ii）在人血中具有活性。尽管酿酒酵母适合于HRPL的定向进化，但是在该宿主中获得的分泌水平还不足以用于进一步的研究和开发。因此，寻找替代宿主以过度表达进化的漆酶是必须的。
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