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Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei

机译:比较转录组分析揭示了黑曲霉和里氏木霉降解蒸汽爆破甘蔗渣的不同策略

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摘要

BackgroundSecond generation (2G) ethanol is produced by breaking down lignocellulosic biomass into fermentable sugars. In Brazil, sugarcane bagasse has been proposed as the lignocellulosic residue for this biofuel production. The enzymatic cocktails for the degradation of biomass-derived polysaccharides are mostly produced by fungi, such as Aspergillus niger and Trichoderma reesei. However, it is not yet fully understood how these microorganisms degrade plant biomass. In order to identify transcriptomic changes during steam-exploded bagasse (SEB) breakdown, we conducted a RNA-seq comparative transcriptome profiling of both fungi growing on SEB as carbon source.
机译:背景技术第二代(2G)乙醇是通过将木质纤维素生物质分解为可发酵糖来生产的。在巴西,已经提出将甘蔗渣作为该生物燃料生产的木质纤维素残余物。用于降解源自生物质的多糖的酶混合物主要由真菌产生,例如黑曲霉和里氏木霉。但是,尚未完全了解这些微生物如何降解植物生物量。为了鉴定在蒸汽爆炸的甘蔗渣(SEB)分解过程中的转录组变化,我们对SEB上生长的两种真菌作为碳源进行了RNA-seq比较转录组分析。

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