RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3 SNP-in-primer and mis-priming from structural variation

摘要

BackgroundPolymerase chain reaction (PCR) is very useful in many areas of molecular biology research. It is commonly observed that PCR success is critically dependent on design of an effective primer pair. Current tools for primer design do not adequately address the problem of PCR failure due to mis-priming on target-related sequences and structural variations in the genome.