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A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.)

机译:转录组分析揭示了吲哚GLS连接的生长素生物合成在油菜(Brassica napus L.)的次级休眠中的作用。

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摘要

BackgroundBrassica napus L. has little or no primary dormancy, but exhibits great variation in secondary dormancy. Secondary dormancy potential in oilseed rape can lead to the emergence of volunteer plants that cause genetic contamination, reduced quality and biosafety issues. However, the mechanisms underlying secondary dormancy are poorly understood. In this study, cultivars Huaiyou-WSD-H2 (H) and Huaiyou-SSD-V1 (V), which exhibit low (approximately 5%) and high (approximately 95%) secondary dormancy rate, respectively, were identified. Four samples, before (Hb and Vb) and after (Ha and Va) secondary dormancy induction by polyethylene glycol (PEG), were collected to identify the candidate genes involved in secondary dormancy via comparative transcriptome profile analysis.
机译:背景甘蓝型油菜(Brassica napus L.)几乎没有或根本没有休眠,但在次生休眠中表现出很大的差异。油菜的二次休眠潜能可能导致志愿植物的出现,这些志愿植物引起遗传污染,质量下降和生物安全问题。但是,对次级休眠的机制了解得很少。在这项研究中,确定了分别表现出低(大约5%)和高(大约95%)次生休眠率的品种Huaiyou-WSD-H2(H)和Huaiyou-SSD-V1(V)。通过比较转录组谱分析,收集了四个样品,分别通过聚乙二醇(PEG)诱导(Hb和Vb)和(Ha和Va)进行第二次休眠,以鉴定参与第二次休眠的候选基因。

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