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Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea

机译:RNA-Seq对经济上重要的油菜作物芥菜的高温和干旱胁迫调控基因的全球见解

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摘要

BackgroundBrassica juncea var. Varuna is an economically important oilseed crop of family Brassicaceae which is vulnerable to abiotic stresses at specific stages in its life cycle. Till date no attempts have been made to elucidate genome-wide changes in its transcriptome against high temperature or drought stress. To gain global insights into genes, transcription factors and kinases regulated by these stresses and to explore information on coding transcripts that are associated with traits of agronomic importance, we utilized a combinatorial approach of next generation sequencing and de-novo assembly to discover B. juncea transcriptome associated with high temperature and drought stresses.
机译:背景芸苔芥菜Varuna是十字花科的一种经济上重要的油料作物,在其生命周期的特定阶段易受非生物胁迫的影响。迄今为止,尚未尝试阐明其全基因组转录组中针对高温或干旱胁迫的变化。为了获得对这些压力调控的基因,转录因子和激酶的全球了解,并探索与农艺学重要性状相关的编码转录本的信息,我们采用了下一代测序和重组组装的组合方法来发现芥菜型油菜。与高温和干旱胁迫相关的转录组。

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