Defining reference genes in Oryza sativa using organ development biotic and abiotic transcriptome datasets

摘要

BackgroundReference genes are widely used to normalise transcript abundance data determined by quantitative RT-PCR and microarrays. However, the approaches taken to define reference genes can be variable. Although Oryza sativa (rice) is a widely used model plant and important crop specie, there has been no comprehensive analysis carried out to define superior reference genes.