A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

摘要

BackgroundMicrospore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolated microspores at 32°C is considered to be a pre-requisite for embryogenesis induction.