BackgroundPostharvest ripening of apple (Malus x domestica) can be slowed down by low temperatures, and a combination of low O2 and high CO2 levels. While this maintains the quality of most fruit, occasionally storage disorders such as flesh browning can occur. This study aimed to explore changes in the apple transcriptome associated with a flesh browning disorder related to controlled atmosphere storage using RNA-sequencing techniques. Samples from a browning-susceptible cultivar (‘Braeburn’) were stored for four months under controlled atmosphere. Based on a visual browning index, the inner and outer cortex of the stored apples was classified as healthy or affected tissue.
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机译：背景技术低温以及低O2和高CO2的结合会降低苹果（Malus x domestica）的收获后成熟速度。尽管这可以保持大多数水果的品质，但偶尔也会发生存储异常，例如果肉褐变。这项研究旨在探讨与使用RNA测序技术控制气调贮藏有关的果肉褐变障碍相关的苹果转录组的变化。褐变敏感品种（“ Braeburn”）的样品在受控气氛下保存了四个月。根据视觉上的褐变指数，将存储的苹果的内部和外部皮层分类为健康或受影响的组织。
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