A perforated anodised aluminium slide for improved specimen clearing and imaging for confocal laser scanning microscopy

摘要

ObjectiveThe bleaching, clearing and handling of tiny specimens with soft tissue and cuticular components for confocal laser scanning microscopy is difficult, because after cuticle bleaching and tissue clearing the specimens are virtually invisible. We have adjusted the design of the specimen container described by Smolla et al. (Arthropod Struct Dev 43:175–81, ) to handle tiny specimens.