CONFOCAL LASER SCANNING MICROSCOPY APPLYING TWO DIRECTION LINE SCANNING WAY AND IMAGE PROCESSING METHOD USING THEREOF

摘要

The present invention is a light source, a laser beam emitted from the light source for irradiating a laser beam horizontally and vertically a first polarization beam splitter for separating a polarized light beam, the first and the second phase difference plate that converts each separated horizontally and vertically polarized beam from the first polarizing beam splitter into circularly polarized light beam, said first and second retardation and the first and second lens for outputting different types of beam focus line of the two perpendicular directions with a converted from a circularly polarized beam, each plate, the first and each output in the form of a line focus beam from the second lens and the first and second scanners for scanning in the vertical direction by changing the path, the beam splitter to convert the path to each of the scanning beam in the process direction from the first sample and the second scanner, between the beam splitter and the sample is disposed, and an objective lens for focusing the incident beam, are respectively disposed between the first polarizing beam splitter and the first and second retarder, is reflected by the sample coming back along the incident light path to the first reflection By including the second and third polarized beam splitter to convert the beam path is advanced in a first direction and a second image pickup device, high-speed image capture speed and there is an effect that can have the advantage of high resolution at the same time.