RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts

摘要

BackgroundThe quantitative polymerase chain reaction (qPCR) is a widely utilized method for gene-expression analysis. However, insufficient material often compromises large-scale gene-expression studies. The aim of this study is to evaluate an RNA pre-amplification method to produce micrograms of cDNA as input for qPCR.