LightCycler RNA Pre-Amplification Kit is a Highly Effective Tool for Gene Expression Studies in Scarce and Fragmented RNA Samples

摘要

There is growing interest in gene expression studies of clinical tissue or blood samples to identify new molecular targets, better molecular diagnostic tools and biomarkers which predict drug efficacy. The problem is that clinical samples are available in only limited size and quantity and generally cannot be replaced from the same individual once the whole sample has been processed. These samples also often contain limited quantity and insufficient quality mRNA. Therefore, the amplified cDNA pool must typically be produced from ultra small amounts of mRNA.