A Biophysic Médical Nanolas Q-Switched pulsed neodymium-YAG laser has been used to produce lesions in human trabecular meshwork in vitro. Sectors of corneoscleral tissue containing trabecular meshwork were suspended in a waterbath which had had a Trokel gonioscopic contact lens mounted into one side by means of a watertight seal. The laser was used to produce lesions in the trabecular meshwork on either side of each specimen. The energy levels delivered ranged from 0.5 to 7.0 mJ, and convergence angles of 10 degrees and 18 degrees were employed. The energy levels required to produce discrete lesions into the canal of Schlemm without perforating the underlying sclera were 3.0-5.0 mJ at the 10 degrees convergence angle and 4.0-6.0 mJ at the 18 degrees convergence angle setting. It is recommended that such data be determined for each type of laser prior to attempting short-pulsed laser internal trabeculotomy in patients with glaucoma.
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