1 Cross-reactivity of ergot alkaloids with an antiserum produced against lysergic acid conjugated with human serum, albumin was utilized to develop a radioimmunoassay for ergotamine, dihydroergotamine, dihydroergotoxine, ergometrine and methylergometrine in biological fluids. The antisera showed no cross-reactivity with simpler indole structures. 2 A procedure for extraction and concentration of alkaloids in biological fluids was developed. 3 The assay is sensitive for 1.8 ng/ml ergotamine, 1.5 ng/ml dihydroergotamine, 2.2 ng/ml dihydroergotoxine, 0.7 ng/ml ergotmetrine and 0.5 ng/ml methylergometrine. 4 The assay is sufficiently sensitive to permit the measurement of urine and plasma ergot alkaloid levels and it is suitable for determination in cases where a known ergot alkaloid is used.
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机译：1麦角生物碱与针对与人血清缀合的麦角酸的抗血清产生的抗血清的交叉反应性，白蛋白被用于开发生物流体中麦角胺，二氢麦角胺，二氢麦角毒素，麦角新碱和甲基麦角新碱的放射免疫分析法。抗血清与简单的吲哚结构无交叉反应。 2开发了一种提取和浓缩生物体液中生物碱的程序。 3该测定法对1.8 ng / ml麦角胺，1.5 ng / ml二氢麦角胺，2.2 ng / ml二氢麦角毒素，0.7 ng / ml麦角紫杉醇和0.5 ng / ml甲基麦角新碱敏感。 4该测定法足够灵敏，可以测量尿和血浆麦角生物碱的水平，适用于使用已知麦角生物碱的情况下的测定。
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