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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

机译：评估通过两种技术从结核分枝杆菌中提取的dna的质量以快速进行分子鉴定和基因分型

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摘要

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

机译：我们报告了两种DNA提取技术，热解和化学裂解（CTAB），用于结核分枝杆菌的分子鉴定和基因分型的比较研究。对40个DNA样品进行了PCR，结果表明，通过热解可以得到有用的数据，从而能够快速鉴定和基因分型。

著录项

期刊名称 Brazilian Journal of Microbiology
作者
Marcelo Miyata; Adolfo Carlos Barreto Santos; Natália Helena Mendes; Eunice Atsuko Cunha; Fernando Augusto Fiúza de Melo; Clarice Queico Fujimura Leite;
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作者单位

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年(卷),期 2011(42),2
年度 2011
页码 774–777
总页数 4
原文格式 PDF
正文语种
中图分类微生物学;
关键词
DNA extraction Mycobacterium tuberculosis thermolysis CTAB;

机译：DNA提取;结核分枝杆菌;热解;CTAB;

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专利

1. Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping [J] . Adolfo Carlos Barreto Santos, Clarice Queico Fujimura Leite, Eunice Atsuko Cunha, Brazilian Journal of Microbiology . 2011,第2期

机译：评估通过两种技术从结核分枝杆菌中提取的dna的质量，以快速进行分子鉴定和基因分型
2. Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping [J] . Miyata Marcelo, Santos Adolfo Carlos Barreto, Mendes Natália Helena, Revista de microbiologia . 2011,第2期

机译：评估通过两种技术从结核分枝杆菌中提取的dna的质量，以快速进行分子鉴定和基因分型
3. Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA [J] . Barbara Molina-Moya, Michel K. Gomgnimbou, Lizania Spinasse, PLOS Neglected Tropical Diseases . 2018,第2期

机译：基于从Ziehl-Neelsen染色载玻片提取的DNA进行的寡核苷酸分型获得的在尼日利亚流通的结核分枝杆菌复杂基因型
4. Visualization of tuberculosis patient and Mycobacterium tuberculosis complex genotype data via host-pathogen maps [C] . Bennett Kristin P., Ozcaglar Cagri, Ranganathan Janani, 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops . 2011

机译：通过宿主-病原体图可视化结核病患者和结核分枝杆菌复杂基因型数据
5. Genotyping of Mycobacterium tuberculosis and Mycobacterium leprae Ancient DNA [D] . Kerudin, Ammielle A. 2020

机译：结核分枝杆菌和分枝杆菌古代DNA的基因分型
6. Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA [O] . Barbara Molina-Moya, Michel K. Gomgnimbou, Lizania Spinasse, 2018

机译：基于从Ziehl-Neelsen染色载玻片提取的DNA进行的寡核苷酸分型获得的在尼日利亚流通的结核分枝杆菌复杂基因型
7. Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping [O] . Miyata, Marcelo [UNESP], Santos, Adolfo Carlos Barreto [UNESP], Mendes, Natália Helena, 2011

机译：评估通过两种技术从结核分枝杆菌中提取的dna的质量，以快速进行分子鉴定和基因分型

Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

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