In many health facilities in east Africa, haemoglobin estimation is performed using visual colour comparison methods. Efforts to establish colorimetric methods face numerous constraints, including the unavailability of standards for quality control. In contrast, the alkaline haematin D-575 method for haemoglobin estimation is a colorimetric method that uses primary standards prepared from pure, crystalline chlorohaemin. There is no significant difference in the accuracy of the alkaline haematin D-575 method and that of the reference haemiglobincyanide method (P > 0.05), and the response of the alkaline haematin D-575 method is linear for serially diluted blood samples over the haemoglobin concentration range 19.6-3.3 g/dl (r = 0.994, y = 1.01 x - 0.3). The method has a precision of +/-0.3 g/dl (coefficient of variation = (1.8%) for whole blood, and is suitable for use with fixed-wavelength haemglobinometers (lambda = 565 nm) or with colorimeters at lambda = 580 nm. Stable quality control standards could be prepared at provincial, zonal, or reference laboratories and distributed regularly to outlying laboratories.
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机译：在东非的许多医疗机构中，使用视觉颜色比较方法进行血红蛋白估计。建立比色法的工作面临许多限制，包括无法使用质量控制标准。相反，用于估计血红蛋白的碱性血红素D-575方法是一种比色法，它使用由纯净结晶氯仿血红素制备的一级标准。碱性血红素D-575方法和参考血红蛋白氰化物方法的准确性没有显着差异（P> 0.05），并且碱性血红素D-575方法对连续稀释的血样在血红蛋白上的响应是线性的浓度范围为19.6-3.3 g / dl（r = 0.994，y = 1.01 x-0.3）。该方法的精度为+/- 0.3 g / dl（全血变异系数=（1.8％），适用于固定波长的血红蛋白仪（λ= 565 nm）或色度计在λ= 580 nm时可以在省，地区或参考实验室制定稳定的质量控制标准，并定期分发给外围实验室。
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