1. Ca buffers may be introduced into human red cells by reversible haemolysis. The resealed ghosts retain Ca and chelating anions in the same ratio as in the haemolysing solution, enabling the intracellular Ca2+ concentration to be calculated simply. 2. The passive permeability of the ghosts to Na and Cl is unaffected by intracellular Ca2+ concentrations in the 10(-8)-10(-4) M range, whereas the K permeability is greatly increased at concentrations above 10(-7) M. 3. These preparations enable Ca-dependent K movements to be studied under stable conditions. When the ghosts contain about 5 X 10(-6) M-Ca2+, over 96% of K transport occurs via the Ca-sensitive route.
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机译：1. Ca缓冲液可通过可逆溶血作用引入人红细胞。重新密封的重影保留的钙和螯合阴离子的比例与溶血溶液中的比例相同，从而可以轻松计算细胞内Ca2 +的浓度。 2.幽灵对Na和Cl的被动渗透性不受细胞内Ca（2+）在10（-8）-10（-4）M范围内的影响，而K渗透性在10（-7）M以上的浓度下大大增加。3.这些准备工作可以在稳定条件下研究钙依赖性钾的运动。当鬼影包含大约5 X 10（-6）M-Ca2 +时，超过96％的K转运通过钙敏感途径发生。
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