BackgroundVascular progenitor cells (VPCs) derived from embryonic stem cells (ESCs) are a valuable source for cell- and tissue-based therapeutic strategies. During the optimization of endothelial cell (EC) inductions from mouse ESCs using our staged and chemically-defined induction methods, we found that cell seeding density but not VEGF treatment between 10 ng/mL and 40 ng/mL was a significant variable directing ESCs into FLK1+ VPCs during stage 1 induction. Here, we examine potential contributions from cell-to-cell signaling or cellular metabolism in the production of VPCs from ESCs seeded at different cell densities.
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机译：背景技术源自胚胎干细胞（ESC）的血管祖细胞（VPC）是基于细胞和组织的治疗策略的重要来源。在使用我们分阶段的化学定义的诱导方法优化小鼠ESC的内皮细胞（EC）诱导的过程中，我们发现10 ng / mL至40 ng / mL之间的细胞接种密度而非VEGF处理是将ESC引导至在第1阶段诱导期间，FLK1 + VPC。在这里，我们研究了从细胞间信号传导或细胞代谢在以不同细胞密度接种的ESC产生VPC的潜在贡献。
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