P3 - Human Mesenchymal Stem Cells: Isolation and Concentration from Bone Marrow using the Regenkit Device

摘要

Introduction:The adult stem cells used in clinical practice are: hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). These cells, present in the bone marrow, play a key role in the physiological renewal and repair of injured tissues. MSCs can differentiate into a variety of cell types including bone cells, cartilage cells, connective cells etc., and they play an important role in the repair of tissue injured by trauma or disease. In view of all this, all the technologies for the isolation and the re-implantation, after minimal manipulation, of autologous stem cells are very interesting, also to make the use of stem cell therapy more widespread in the orthopaedic field.The aim of this study was to verify the RegenKit method of selection and enrichment of autologous mesenchymal and hematopoietic stem cell fractions from bone marrow, used intraoperatively, in order to define a standard therapeutic application.