Antibody titres of antilymphocytic sera and globulins determined by immunofluorescence and their correlation with immunosuppressive activity

摘要

Human and mouse lymphocytes have been treated with the appropriate antilymphocytic serum or globulin and the indirect fluorescent technique applied. A method of scoring the observed fluorescence has been devised. A good general degree of correlation has been demonstrated between the antibody titres of the antilymphocytic sera and globulins, determined by immunofluorescence and the immunosuppressive activity as measured by graft prolongation. The test is reliable, rapid and reproducible, but absolute reliance on the test is not advocated as a precise measure of immunosuppressive activity. The immunofluorescent technique is invaluable in the analysis of serum samples from horses undergoing active immunization.