A Laboratory Exercise for a College-Level Introductory Neuroscience Course Demonstrating Effects of Housing Environment on Anxiety and Psychostimulant Sensitivity

摘要

In this paper we describe a lab exercise developed for the Introduction to Neuroscience course at Williams College. One of a series of five labs, this exercise demonstrated several key principles of behavioral neuroscience. In this lab, students explored the effects of post-weaning housing environment on anxiety-like behavior and psychostimulant sensitivity in rodents. The exercise was intended to emphasize the importance and utility of animal models in neuroscience research and to give students hands-on experience with behavioral neuroscience research techniques. Students tested rats reared in social isolation or environmental enrichment for anxiety-like behaviors on the elevated plus maze, and for spontaneous and amphetamine-induced locomotor activity in the open field. They were then asked to analyze pooled class data and prepare a short lab report. Overall, student performance was excellent. This exercise emerged as a class favorite on course evaluations. Interestingly, the first time this exercise was conducted, the effects of environmental enrichment on anxiety-like behaviors and psychostimulant sensitivity were not consistent with those published in previous studies. Key methodological issues that may account for this discrepancy and contribute to successful implementation by other programs are discussed.