Cytokine profiles of suction pulmonary secretions from children infected with pandemic influenza A(H1N1) 2009

摘要

Uncomplicated influenza in humans is characterized by massive virus replication in respiratory epithelial cells, inflammation and an abrupt onset. The novel influenza A (H1N1) 2009 caused an epidemic of critical illness and some patients rapidly developed severe acute respiratory distress syndrome [,]. Van Reeth [] reviewed growing evidence that the so-called early cytokines produced at the site of infection mediate many of the clinical and pathological manifestations of influenza infection. Of those cytokines, Bermejo-Martin and colleagues [] reported in Critical Care that T-helper 1 (Th1) and Th17 hypercytokinemia plays an important role as an early host response in severe pandemic influenza. Evaluating the differences in early immune responses between hospitalized patients with severe pandemic influenza and those with mild disease, high systemic levels of IFN-γ and a group of mediators involved in the development of the Th17 (IL-8, IL-9, IL-17, IL-6) and Th1 (TNF-α, IL-15, IL-12p70) responses were found exclusively in hospitalized patients. A significant inverse association was found between IL-6 and IL-8 and PaO2 in critical patients. They concluded that severe disease with respiratory involvement is characterized by early secretion of Th17 and Th1 cytokines.We experienced two cases of pandemic influenza A(H1N1) 2009-associated pneumonia and encephalopathy, which were treated under mechanical ventilation. Cytokine analysis of their pulmonary secretions revealed different patterns from previous results (Figure ). One patient showed no improvement with usual ventilation and had mediastinal emphysema and serious hypooxygenation; thus, the patient needed to be ventilated using the special respiratory airway pressure release ventilation mode because of progression and the need for high pressure control. The second case with encephalopathy complicated with pneumonia underwent combined treatments of steroids and hypothermia because of intractable recurrent seizures. Their cytokine levels were extremely high, although serum 17 cytokines were within normal ranges. Cytokines in pulmonary secretions at first revealed high levels of IL-8, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein-1b (MIP-1b). On the other hand, IL-1b, 2, 4, 5, 6, 7, 10, 12, 13, 17, G-CSF, GM-CSF, IFN-γ and TNF-α were normal or slightly increased. On 5th days after hospitalization other cytokines (IL-1β, 6, 10, 17, G-CSF, GM-CSF, IFN-γ, MCP-1 and TNF-α) increased markedly in both cases. Given these findings, we suspect that chemokines play a role mostly in lung injury associated with influenza A(H1N1) 2009 infection in the early phase. High levels of chemokines and subsequent epithelial changes will increase the permeability in alveoli and fibrin leakage into interstitial tissue, followed by the consequent production of other inflammatory cytokines. Alternatively, we assume that secondary bacterial infections, which have been reported [], influence the production of those cytokines (IL-6, IL-1b and TNF-α).>Cytokines in the pulmonary secretions of a case with serious distress and oxygenic disturbance (upper panel). >Another case showed almost similar patterns (lower panel). G-CSF, granulocyte colony stimulating factor; GM-CSF, granulocyte-macrophage colony stimulating factor; IFN, interferon; IL, interleukin; MCP, monocyte chemotactic protein; TNF, tumor necrosis factor.