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Transcription profile data of phorbol esters biosynthetic genes during developmental stages in Jatropha curcas

机译:麻疯树发育过程中佛波酯生物合成基因的转录谱数据

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摘要

Jatropha curcas is currently known as an alternative source for biodiesel production. Beside its high free fatty acid content, J. curcas also contains typical diterpenoid-toxic compounds of Euphorbiaceae plant namely phorbol esters. This article present the transcription profile data of genes involved in the biosynthesis of phorbol esters at different developmental stages of leaves, fruit, and seed in Jatropha curcas. Transcriptional profiles were analyzed using reverse transcription-polymerase chain reaction (RT-PCR). We used two genes including GGPPS (Geranylgeranyl diphospate synthase), which is responsible for the formation of common diterpenoid precursor (GGPP) and CS (Casbene Synthase), which functions in the synthesis of casbene. Meanwhile, J. curcas Actin (ACT) was used as internal standard. We demonstrated dynamic of GGPPS and CS expression among different stage of development of leaves, fruit and seed in Jatropha.
机译:麻疯树目前被称为生物柴油生产的替代来源。除了其高的游离脂肪酸含量外,麻疯树还含有大戟科植物中典型的二萜类有毒化合物,即佛波醇酯。本文介绍了麻疯树叶片,果实和种子不同发育阶段参与佛波醇酯生物合成的基因的转录谱数据。使用逆转录-聚合酶链反应(RT-PCR)分析转录谱。我们使用了两个基因,包括负责GPPS合成的通用二萜前体(GGPP)和CS(Casbene合酶)的形成的GGPPS(Geranylgeranyl diphospate合酶)。同时,使用J. curcas Actin(ACT)作为内标。我们证明了麻风树叶片,果实和种子发育的不同阶段中GGPPS和CS表达的动态。

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