Protein kinase C/ζ (PRKCZ) Gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes

摘要

AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed.RESULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P < 0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.CONCLUSION: PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.