Effect of Boschniakia rossica on expression of GST-P p53 and p21ras proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

摘要

AIM: To investigate the effect of Boschniakia rossica (BR) extract on expression of GST-P, p53 and p21^ras proteins in early stage of chemical hepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS: The expression of tumor marker-placental form glutathione S-transferase (GST-P), p53 and p21^ras proteins were investigated by immunohistochemical techniques and ABC method. Anti-inflammatory activities of BR were studied by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet-induced mouse granuloma formation methods.RESULTS: The 500 mg/kg of BR-H2O extract fractionated from BR-Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver (GST-P staining was 78% positive in DEN+AAF group vs 20% positive in DEN+AAF+BR group, P < 0.05) and the expression of mutant p53 and p21^ras protein was lower than that of hepatic preneoplastic lesions (33% and 22% positive respectively in DEN+AAF group vs negative in DEN+AAF+BR group). Both CH2Cl2 and H2O extracts from BR had anti-inflamatory effect in xylene and croton oil-induced mouse ear edema (inhibitory rates were 26%-29% and 35%-59%, respectively). BR-H2O extract exhibited inhibitory effect in carrageenin, histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet-induced granuloma formation in mice.CONCLUSION: BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical-hepato-carcinogenesis. Both CH2Cl2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice.