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Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

机译：定量测定牛病毒性腹泻病毒1型的一步法SYBR Green实时RT-PCR及其与常规RT-PCR的比较

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摘要

BackgroundBovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established for BVDV detection. This study aims to develop a quantitative one-step RT-PCR assay to detect BVDV type-1 in cell culture.

机译：背景牛病毒性腹泻病毒（BVDV）是牛的一种世界性病原体，是丙型肝炎病毒（HCV）的替代模型。尚未建立基于SYBR Green I染料的一步式实时荧光定量逆转录聚合酶链反应（RT-PCR）分析用于BVDV检测。这项研究旨在开发一种定量的一步式RT-PCR分析法，以检测细胞培养物中的BVDV 1型。

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期刊名称 Virology Journal
作者
Ni Zhang; Zhengwen Liu; Qunying Han; Jianming Qiu; Jinghong Chen; Guoyu Zhang; Zhu Li; Sai Lou; Na Li;
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作者单位

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年(卷),期 2011(8),-1
年度 2011
页码 374
总页数 8
原文格式 PDF
正文语种
中图分类人体病毒学（致病病毒）;病毒（滤过性病毒）;
关键词
Bovine viral diarrhea virus type-1 cRNA standard SYBR Green I RT-PCR Quantitation Cell culture;

机译：牛病毒性腹泻病毒1型;cRNA标准;SYBR Green I RT-PCR;定量;细胞培养;

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专利

1. Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR [J] . Ni Zhang, Zhengwen Liu, Qunying Han, Virology Journal . 2011,第1期

机译：定量测定牛病毒性腹泻病毒1型的一步法SYBR Green实时RT-PCR及其与常规RT-PCR的比较
2. Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches [J] . Zhang Shu-Qin, Tan Bin, Li Peng, Journal of Virological Methods . 2014,第Null期

机译：常规RT-PCR，逆转录环介导的等温扩增和基于SYBR Green I的实时RT-PCR在快速检测受污染的商业牛血清批次中的牛病毒性腹泻病毒核苷酸中的比较
3. Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches [J] . Zhang Shu-Qin, Tan Bin, Li Peng, Journal of Virological Methods . 2014,第Null期

机译：常规RT-PCR，逆转录环介导的等温扩增和基于SYBR Green I的实时RT-PCR在快速检测受污染的商业牛血清批次中的牛病毒性腹泻病毒核苷酸中的比较
4. DEVELOPMENT OF A MINOR GROOVE BINDER ASSAY FOR SENSITIVE REAL-TIME ONE-STEP RT-PCR DECTECTION OF SWINE VESICULAR DISEASE VIRUS [C] . Michael J. McMcnamy, John McKillen, Bemt Hjertner, Congress ofthe International Pig Veterinary Society . 2008

机译：开发次沟粘合剂测定的敏感实时一步RT-PCR Dectection的猪尿布疾病病毒
5. Real-time RT-PCR of feline calicivirus and optimization for detection of virus in feline urine. [D] . Scansen, Brian Alan. 2004

机译：猫杯状病毒的实时RT-PCR和优化检测猫尿中病毒的方法。
6. Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco [O] . Siham Fellahi, Mehdi El Harrak, Jens H. Kuhn, 2016

机译：SYBR green I实时RT-PCR与基于常规琼脂糖凝胶的RT-PCR在摩洛哥鸡中诊断传染性支气管炎病毒感染的比较
7. Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR [O] . Lou Sai, Li Zhu, Zhang Guoyu, 2011

机译：定量测定牛病毒性腹泻病毒1型的一步法SYBR Green实时RT-PCR及其与常规RT-PCR的比较

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

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