Microbiota of Palm Oil Mill Wastewater in Malaysia

摘要

This study was aimed at identifying indigenous microorganisms from palm oil mill effluent (POME) and to ascertain the microbial load. Isolation and identification of indigenous microorganisms was subjected to standard microbiological methods and sequencing of the 16S rRNA and 18S rRNA genes. Sequencing of the 16S rRNA and 18S rRNA genes for the microbial strains signifies that they were known as Micrococcus luteus 101PB, Stenotrophomonas maltophilia 102PB, Bacillus cereus 103PB, Providencia vermicola 104PB, Klebsiella pneumoniae 105PB, Bacillus subtilis 106PB, Aspergillus fumigatus 107PF, Aspergillus nomius 108PF, Aspergillus niger 109PF and Meyerozyma guilliermondii 110PF. Results revealed that the population of total heterotrophic bacteria (THB) ranged from 9.5 × 10⁵ – 7.9 × 10⁶ cfu/mL. The total heterotrophic fungi (THF) ranged from 2.1 × 10⁴ – 6.4 × 10⁴ cfu/mL. Total viable heterotrophic indigenous microbial population on CMC agar ranged from 8.2 × 10⁵ – 9.1 × 10⁶ cfu/mL and 1.4 × 10³ – 3.4 × 10³ cfu/mL for bacteria and fungi respectively. The microbial population of oil degrading bacteria (ODB) ranged from 6.4 × 10⁵ – 4.8 × 10⁶ cfu/mL and the oil degrading fungi (ODF) ranged from 2.8 × 10³ – 4.7 × 10⁴ cfu/mL. The findings revealed that microorganisms flourish well in POME. Therefore, this denotes that isolating native microorganisms from POME is imperative for effectual bioremediation, biotreatment and biodegradation of industrial wastewaters.