FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITALIN TRIANGULO MINEIRO MINAS GERAIS STATE BRAZIL

摘要

Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitroresistance to azoles was not detected, except for C. krusei. The two primers,OPA09 and OPB11, were able to distinguish different species. Isolates of C.albicans and C. parapsilosis complex species presentedsix and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing thegenetic variability of the isolates of those species. It was concluded that membersof the C. parapsilosis complex were the most frequent species found,and most isolates were susceptible to the antifungals amphotericin B, flucozanole,and itraconazole. High genetic polymorphisms were observed for isolates of C.albicans and C. parapsilosis complex species, mainlywith the OPA09 marker.