A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.
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机译：开发了一种侧向流免疫色谱带测试技术，可通过胶体金标记抗HES单克隆抗体对鱼样品中的己烯雌酚（HES）残留物进行快速而灵敏的现场检测。该试纸条由样品垫，结合试剂垫，吸收剂垫和包含控制线和测试线的测试膜组成。确定鱼提取物样品的检测条的灵敏度（半抑制浓度，IC50）为1.86 µg kg -1 ，检测值的下限为0.62 µg kg - 1 。对于测定内和测定间可重复性，加标的HES样品的回收率分别为86.3％至92.3％和85.8％至93.4％，变异系数分别为2.91–4.64％和4.24–5.17％。采用高效液相色谱法确认试纸的性能。剥离测试花费不到10分钟，因此提供了一种用于现场检测HES残留物的有偿方法。
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