BackgroundA large variety of proteins involved in inflammation, coagulation, lipid-oxidation and lipid metabolism have been associated with high-density lipoprotein (HDL) and it is anticipated that changes in the HDL proteome have implications for the multiple functions of HDL. Here, SELDI-TOF mass spectrometry (MS) was used to study the dynamic changes of HDL protein composition in a human experimental low-dose endotoxemia model. Ten healthy men with low HDL cholesterol (0.7+/-0.1 mmol/L) and 10 men with high HDL cholesterol levels (1.9+/-0.4 mmol/L) were challenged with endotoxin (LPS) intravenously (1 ng/kg bodyweight). We previously showed that subjects with low HDL cholesterol are more susceptible to an inflammatory challenge. The current study tested the hypothesis that this discrepancy may be related to differences in the HDL proteome.
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机译：背景技术与炎症，凝血，脂质氧化和脂质代谢有关的多种蛋白质已与高密度脂蛋白（HDL）相关联，并且预计HDL蛋白质组的变化会影响HDL的多种功能。在这里，SELDI-TOF质谱（MS）用于研究人类实验性低剂量内毒素血症模型中HDL蛋白组成的动态变化。接受10 ng HDL胆固醇（0.7 +/- 0.1 mmol / L）的健康男性和10例HDL胆固醇水平高（1.9 +/- 0.4 mmol / L）的男性静脉内毒素（LPs）攻击（1 ng / kg体重） 。我们之前的研究表明，低HDL胆固醇的受试者更容易发炎。当前的研究检验了这种差异可能与HDL蛋白质组差异有关的假设。
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