An antiserum generated in rabbits against human seminal alpha-inhibin-52 has been used to develop a sensitive radioimmunoassay for the detection of alpha-inhibins. The alpha-inhibin-52 antiserum reacts with alpha-inhibin-92 and alpha-inhibin-31 with equal avidity. These peptides were found to be present in human pituitary, hypothalamus, and serum. In exclusion chromatography on Sephadex G-100, the immunoreactive material eluted in a large molecular size region. Immunoblot analysis of column-derived fractions of these extracts revealed the presence of alpha-inhibin-92. The mean concentrations of immunoreactive alpha-inhibin were found to be 7.2 ng/ml in normal adult male serum, 70.3 ng/g (wet weight) of pituitary, and 12.9 ng/g (wet weight) of hypothalamus. This communication reports on the evidence for the existence of gonadal peptides in the brain.
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机译:在兔体内产生的针对人类精浆α-抑制素-52的抗血清已用于开发灵敏的放射免疫分析法,以检测α-抑制素。 α-抑制素52抗血清与α-抑制素92和α-抑制素31具有相同的亲和力。发现这些肽存在于人垂体,下丘脑和血清中。在Sephadex G-100上的排阻色谱法中,免疫反应性材料在大分子区域内洗脱。这些提取物的柱衍生级分的免疫印迹分析表明存在α-抑制素-92。发现正常成年男性血清中免疫反应性α-抑制素的平均浓度为7.2 ng / ml,垂体为70.3 ng / g(湿重),下丘脑为12.9 ng / g(湿重)。该通讯报道了大脑中存在性腺肽的证据。
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