Analysis of the effects of forelimb amputation and denervation on [35S]methionine incorporation into the protein of newt brachial plexus nerve ganglia showed that amputation increases the level of protein synthesis about 4-fold as compared with that of control (nonamputated) animals. Denervation without amputation nearly doubles the level of protein synthesis as compared with controls. Analysis of labeled protein by means of two-dimensional gel electrophoresis and radioautogram revealed incorporation patterns that are similar to controls; this observation suggests that amputation affects nerve cells quantitatively rather than qualitatively, in influencing the production of putative neurotrophic agents. A group of basic proteins ranging in Mr from 15,000 to 31,000 are prominently expressed in these radioautograms from experimental ganglia and may be important in promoting forelimb regeneration in the newt.
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