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The Gibberellic Acid Stimulated-Like Gene Family in Maize and Its Role in Lateral Root Development

机译:玉米赤霉素样基因家族及其在侧根发育中的作用

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摘要

In an approach to study lateral root development in monocots, genome-wide searches for homologs of the Gibberellic Acid Stimulated Transcript-like (GAST-like) gene family in rice (Oryza sativa) and maize (Zea mays) were carried out. Six novel GAST-like genes in rice and 10 members of the gene family in maize, which were designated ZmGSL (for Z. mays Gibberellic Acid Stimulated-Like), were identified. The ZmGSL family encodes small proteins of 75 to 128 amino acids, which are characterized by a conserved 59 to 64 amino acid C-terminal domain. Within this domain, 17 amino acids, including 12 cysteines, are perfectly conserved. The transcript of the ZmGSL1 gene is differentially spliced into the alternative variants ZmGSL1a and ZmGSL1b, the latter of which is translated into a premature protein that lacks the C-terminal domain. The presence of an additional N-terminal cleavable signal sequence in eight of the 10 ZmGSL proteins suggests that they are secreted into the extracellular matrix. In-depth root-specific gene expression analyses carried out in the wild type and the lateral root mutants lrt1 and rum1 suggest a role for ZmGSL genes in early lateral root development, which is likely regulated by gibberellic acid. Expression patterns of ZmGSL1a and ZmGSL1b propose antagonistic functions of these splice variants during early lateral root formation.
机译:在研究单子叶植物侧根发育的一种方法中,进行了全基因组搜索,以寻找水稻(Oryza sativa)和玉米(Zea mays)中赤霉素刺激的转录样(GAST-like)基因家族的同源物。鉴定了水稻中的六个新的GAST样基因和玉米中的10个基因家族成员,它们被命名为ZmGSL(对于Z. mays赤霉素刺激)。 ZmGSL家族编码75至128个氨基酸的小蛋白质,其特征是保守的59至64个氨基酸的C末端结构域。在该结构域内,包括12个半胱氨酸在内的17个氨基酸被完全保守。 ZmGSL1基因的转录本被剪接到不同的变体ZmGSL1a和ZmGSL1b中,后者被翻译成缺少C端结构域的过早蛋白质。 10个ZmGSL蛋白中有8个存在额外的N端可裂解信号序列,这表明它们被分泌到细胞外基质中。在野生型和侧根突变体lrt1和rum1中进行的深入根特异性基因表达分析表明,ZmGSL基因在早期侧根发育中可能发挥了作用,这可能受赤霉素调节。 ZmGSL1a和ZmGSL1b的表达模式提出了这些剪接变体在早期侧根形成过程中的拮抗作用。

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