The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone), maize (Zea mays [L.] Pioneer 3148), pea (Pisum sativum [L.] cv Progress 9), and soybean (Glycine max [L.] Merr. cv Ransom 2). Northern blot analysis revealed that pea LHC-II mRNA was present in dark-grown seedlings and accumulated rapidly within 1 hour following illumination with white light. In contrast, the accumulation of LHC-II mRNA was delayed in barley and soybean until 2 to 4 hours after illumination began. Single radial immunodiffusion analysis revealed that LHC-II polypeptides began to accumulate in all species between 4 and 8 hours although the protein was present in detectable levels at earlier times in certain species. In a pattern similar to the LHC-II protein accumulation, chlorophyll accumulated at increased rates between 4 and 8 hours of greening in all species following an initial delay. The absence of coordination between LHC-II mRNA and LHC-II protein accumulation that was clearly observed in pea suggested that transcription is not the factor that limits LHC-II complex formation during chloroplast development. The accumulation of chlorophyll and LHC-II protein appeared to coincide suggesting that chlorophyll biosynthesis may be a factor that limits LHC-II complex formation.
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机译:叶绿素/壳聚糖/小球藻/铁/钙/ DHA / EPA /含绿豆/黑豆/红豆/白豆室温暴露,绿豆芽/绿色寄主豆芽/大白菜/新鲜/羽衣甘蓝/马铃薯/大蒜/姜/生姜/黄瓜/南瓜/菠菜/人参/红鸭/迷你番茄/西瓜/瓜,嫩芽/马铃薯/甘薯/黄瓜/西瓜/瓜/大白菜/大蒜/生姜/普通大米,阿克巴里,大米/大米/大麦/荞麦/橡子塑料空气分配孔,把手,阳光遮蔽的盖子,水分配孔处理幼苗,处理水耕栽培容器,不良,岩石形成,固体培养基,水耕,稻田,田地,耕种