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Identification of Maize Kernel Vigor under Different Accelerated Aging Times Using Hyperspectral Imaging

机译:利用高光谱成像技术鉴定不同加速老化时间下玉米籽粒活力

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摘要

Seed aging during storage is irreversible, and a rapid, accurate detection method for seed vigor detection during seed aging is of great importance for seed companies and farmers. In this study, an artificial accelerated aging treatment was used to simulate the maize kernel aging process, and hyperspectral imaging at the spectral range of 874–1734 nm was applied as a rapid and accurate technique to identify seed vigor under different accelerated aging time regimes. Hyperspectral images of two varieties of maize processed with eight different aging duration times (0, 12, 24, 36, 48, 72, 96 and 120 h) were acquired. Principal component analysis (PCA) was used to conduct a qualitative analysis on maize kernels under different accelerated aging time conditions. Second-order derivatization was applied to select characteristic wavelengths. Classification models (support vector machine−SVM) based on full spectra and optimal wavelengths were built. The results showed that misclassification in unprocessed maize kernels was rare, while some misclassification occurred in maize kernels after the short aging times of 12 and 24 h. On the whole, classification accuracies of maize kernels after relatively short aging times (0, 12 and 24 h) were higher, ranging from 61% to 100%. Maize kernels with longer aging time (36, 48, 72, 96, 120 h) had lower classification accuracies. According to the results of confusion matrixes of SVM models, the eight categories of each maize variety could be divided into three groups: Group 1 (0 h), Group 2 (12 and 24 h) and Group 3 (36, 48, 72, 96, 120 h). Maize kernels from different categories within one group were more likely to be misclassified with each other, and maize kernels within different groups had fewer misclassified samples. Germination test was conducted to verify the classification models, the results showed that the significant differences of maize kernel vigor revealed by standard germination tests generally matched with the classification accuracies of the SVM models. Hyperspectral imaging analysis for two varieties of maize kernels showed similar results, indicating the possibility of using hyperspectral imaging technique combined with chemometric methods to evaluate seed vigor and seed aging degree.
机译:储存过程中的种子老化是不可逆的,对于种子公司和农民来说,快速,准确的种子老化过程中的种子活力检测方法至关重要。在这项研究中,使用人工加速老化处理来模拟玉米仁的老化过程,并在874-1734 nm光谱范围内使用高光谱成像技术作为快速准确的技术来鉴定不同加速老化时间制度下的种子活力。获取了两个玉米品种的高光谱图像,这些玉米品种经过八个不同的老化持续时间(0、12、24、36、48、72、96和120 h)处理。使用主成分分析(PCA)对不同加速老化时间条件下的玉米籽粒进行定性分析。应用二阶导数选择特征波长。建立了基于全光谱和最佳波长的分类模型(支持向量机-SVM)。结果表明,未经处理的玉米粒发生误分类的情况很少,而在12和24小时的短时效后,玉米粒发生了一些误分类。总体而言,相对较短的老化时间(0、12和24 h)后,玉米粒的分类精度较高,范围为61%至100%。老化时间较长(36、48、72、96、120小时)的玉米粒分类精度较低。根据SVM模型的混淆矩阵结果,每种玉米品种的八类可分为三类:第1组(0 h),第2组(12和24 h)和第3组(36、48、72, 96,120 h)。一组中不同类别的玉米粒彼此之间更可能被错误分类,而不同组中的玉米粒具有较少的错误分类样本。进行发芽试验以验证分类模型,结果表明,标准发芽试验显示的玉米籽粒活力的显着差异通常与SVM模型的分类精度相符。对两个玉米粒品种的高光谱成像分析显示出相似的结果,表明使用高光谱成像技术结合化学计量学方法评估种子活力和种子老化程度的可能性。

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