The combination of the ionic liquid co-lyophilized lipase and microwave irradiation was used to improve enzyme performance in enantioselective esterification of α-lipoic acid. Effects of various reaction conditions on enzyme activity and enantioselectivity were investigated. Under optimal condition, the highest enantioselectivity (E = 41.2) was observed with a high enzyme activity (178.1 μmol/h/mg) when using the ionic liquid co-lyophilized lipase with microwave assistance. Furthermore, the ionic liquid co-lyophilized lipase exhibited excellent reusability under low power microwave.
展开▼
机译：离子液体共冻干的脂肪酶和微波辐射的组合被用于改善α-硫辛酸的对映选择性酯化中的酶性能。研究了各种反应条件对酶活性和对映选择性的影响。在最佳条件下，当将离子液体共冻干脂肪酶与微波辅助一起使用时，观察到最高的对映选择性（E = 41.2），且酶活性高（178.1μmol/ h / mg）。此外，离子液体共冻干脂肪酶在低功率微波下显示出优异的可重复使用性。
展开▼
