Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells

摘要

Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the isolation of seven phenolic compounds: protoaphin-fb (>1), 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-β-d-glucose (>2), 1,2,3,4,6-penta-O-galloyl-β-d-glucose (>3), 1,2,4,6-tetra-O-galloyl-β-d-glucose (>4), 3-hydroxy-5-methoxy-phenol 1-O-β-d-glucoside (>5), methylgallate (>6), and gallic acid (>7). Their structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and compounds >1-5 exhibited potent inhibitory effects on this enzyme, with IC50 values ranging from 30.6 ± 2.4 to 3.5 ± 0.5 μM. In addition, the highly galloylated compound >2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells.