The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.
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机译：这项研究的目的是评估考虑到Wistar大鼠体内的总辐射量（TBI），黑葡萄汁（BGJ）对脂质过氧化的潜在积极作用。作为原位评估的潜在可行方法，评估了血清乳酸脱氢酶（LDH）水平作为急性放射综合症（ARS）对心脏造成损害的标志。将二十只大鼠分成四组，其中两组用Co-60来源的伽马射线辐照。对动物进行安乐死后，每天用2 mL BGJ或安慰剂管饲动物，进行6 Gy全身γ射线照射前一周和4天后。评估血清中的LDH和心脏组织上的脂质过氧化。心脏中脂质过氧化引起的代谢物浓度高，血清中LDH水平高，仅在接受安慰剂的γ辐照组中出现，主要是在辐照后的最初24小时。 BGJ的植物化学分析是通过测定总酚，类黄酮和单宁酸，然后进行高效液相色谱（HPLC / DAD）分析来进行的，该分析表明白藜芦醇是主要成分。结果表明，BGJ是对抗ARS引起的心脏损害的良好保护候选化合物，其作用表明其可用作放射修饰剂。
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