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WHO Collaborating Centre for Acquired Immunodeficiency Syndrome for the Eastern Mediterranean Regional Office Faculty of Medicine Kuwait University Kuwait

机译:世卫组织科威特大学医学院东地中海区域办事处后天免疫机能丧失综合症合作中心

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摘要

In the early 1980s, the World Health Organization (WHO) designated the Virology Unit of the Faculty of Medicine, Health Sciences Centre, Kuwait University, Kuwait, a collaborating centre for AIDS for the Eastern Mediterranean Regional Office (EMRO), recognizing it to be in compliance with WHO guidelines. In this centre, research integral to the efforts of WHO to combat AIDS is conducted. In addition to annual workshops and symposia, the centre is constantly updating and renewing its facilities and capabilities in keeping with current and latest advances in virology. As an example of the activities of the centre, the HIV-1 RNA viral load in plasma samples of HIV-1 patients is determined by real-time PCR using the AmpliPrep TaqMan HIV-1 test v2.0. HIV-1 drug resistance is determined by sequencing the reverse transcriptase and protease regions on the HIV-1 pol gene, using the TRUGENE HIV-1 Genotyping Assay on the OpenGene® DNA Sequencing System. HIV-1 subtypes are determined by sequencing the reverse transcriptase and protease regions on the HIV-1 pol gene using the genotyping assays described above. A fundamental program of Kuwait's WHO AIDS collaboration centre is the national project on the surveillance of drug resistance in human deficiency virus in Kuwait, which illustrates how the centre and its activities in Kuwait can serve the EMRO region of WHO.
机译:在1980年代初期,世界卫生组织(WHO)指定了科威特科威特大学健康科学中心医学系的病毒学部门,这是东地中海区域办事处(EMRO)艾滋病合作中心。符合WHO准则。在这个中心,进行了世卫组织抗击艾滋病工作不可或缺的研究。除年度研讨会和专题讨论会外,该中心还根据病毒学的最新进展不断更新和更新其设施和能力。作为该中心活动的一个示例,使用AmpliPrep TaqMan HIV-1测试v2.0通过实时PCR测定HIV-1患者血浆样本中的HIV-1 RNA病毒载量。使用OpenGene®DNA测序系统上的TRUGENE HIV-1基因分型测定法,通过对HIV-1 pol基因上的逆转录酶和蛋白酶区域进行测序来确定HIV-1耐药性。通过使用上述基因分型测定法对HIV-1 pol基因上的逆转录酶和蛋白酶区域进行测序,可以确定HIV-1亚型。科威特世界卫生组织艾滋病合作中心的一项基本计划是监测科威特人类缺乏病毒耐药性的国家项目,该项目说明了该中心及其在科威特的活动如何为世界卫生组织急救区域服务。

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