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Studies on Liquefaction Time and Proteins Involved in the Improvement of Seminal Characteristics in Dromedary Camels (Camelus dromedarius)

机译:液化时间和蛋白质参与改善骆驼(骆驼)精子特性的研究

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摘要

Semen was collected from six dromedary camels using artificial vagina during rutting season. Liquefaction of the viscous semen occurred in 23.89 ± 1.49 h. During liquefaction, proteins with molecular masses of 24.55 kDa and 22.07 kDa appeared in conjunction with the disappearance of intact 26.00 kDa protein after 18–24 h. These proteins were identified as β-nerve growth factors (β-NGFs) in liquefied camel semen. Guanidine-HCL improves the rheological characteristics of dromedary camel semen along with significant (P < 0.01) increase in sperm motility. No significant differences were found in viability of spermatozoa indicating no visible detrimental effects on spermatozoa. The cause of semen viscosity, as well as proteins that are present in liquefied dromedary camel seminal plasma, is described for the first time.
机译:在车辙季节,使用人工阴道从六个单峰骆驼中收集精液。粘性精液的液化发生在23.89±1.49 h。在液化过程中,分子量为24.55 kDa和22.07 kDa的蛋白质与完整的26.00 afterkDa蛋白质在18-24Dah后消失有关。这些蛋白被鉴定为液化骆驼精液中的β-神经生长因子(β-NGFs)。胍盐酸盐可改善单峰骆驼精液的流变特性,并显着提高(P <0.01)精子活力。在精子的生存能力上没有发现显着差异,表明对精子没有可见的有害影响。第一次描述了精液粘度以及液化单峰骆驼精浆中存在的蛋白质的原因。

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