A new compound, rotenoid isoflavone glycoside named, 6′-O-β-d-glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fractions of Amorpha fruticosa LINNE. Both of the fractions and purified single compound showed a 19% relatively low cytotoxicity at a maximum concentration of 1.0 g/L in a cultivated normal human lung cell line (HEL299). The purified single compound showed less cytotoxicity than the crude extracts, possibly because residual toxicants were eliminated during purification processes. Cell growth of human T cells was increased by about 15% by adding 0.5 g/L of the fractions compared to the control. Specific production rates of interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) from T cell were higher as 1.16 × 10−4 and 1.86 × 10−4 pg/cell, respectively, in the purified compound, compared to 1.38 × 10−4 and 2.22 × 10−4 pg/cell, respectively, by adding 0.5 g/L of the dichloromethane fraction. Natural killer cell-92MI (NK-92MI) growth supplemented with the supernatant of human T cell was up to 19% higher with the dichloromethane fraction compared with a new single compound at a concentration of 0.5 g/L. Overall, the dichloromethane fraction showed relatively higher immuno-modulatory activities compared with a new single compound, probably due to the synergic effect given by other substances existing in the fractions.
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机译:通过多级柱色谱法从金紫穗槐果实的甲醇(MeOH)果实提取物中分离出一种新的类胡萝卜素异黄酮苷,名为6'-O-β-d-吡喃葡萄糖基-12a-羟基丹酚。将该新糖苷的免疫调节活性与紫穗槐的分配级分进行了比较。在培养的正常人肺细胞系(HEL299)中,最大浓度为1.0 g / L时,馏分和纯化的单一化合物均显示出19%的相对较低的细胞毒性。纯化的单一化合物显示出比粗提取物更低的细胞毒性,这可能是因为在纯化过程中消除了残留的有毒物质。与对照相比,通过添加0.5 g / L的级分,人类T细胞的细胞生长增加了约15%。 T细胞的白细胞介素6(IL-6)和肿瘤坏死因子(TNF-α)的具体生产率较高,分别为1.16×10 -4 sup>和1.86×10 -4 sup>。通过添加0.5 g分别将纯化化合物中的sup> pg / cell与1.38×10 -4 sup>和2.22×10 -4 sup> pg / cell进行比较/ L的二氯甲烷馏分。与浓度为0.5 g / L的新单一化合物相比,补充了人类T细胞上清液的自然杀伤细胞92MI(NK-92MI)的生长与二氯甲烷组分相比提高了19%。总体而言,与新的单一化合物相比,二氯甲烷馏分显示出相对较高的免疫调节活性,这可能是由于馏分中存在的其他物质所产生的协同作用。
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