Asiatic acid (AA), a pentacyclic triterpene compound in the medicinal plant Centella asiatica, was evaluated for antinociceptive and anti-inflammatory effects. Treatment of male ICR mice with AA significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pain in the late phase. In the anti-inflammatory test, AA decreased the paw edema at the 4th and 5th h after λ-carrageenan (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. AA decreased the nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) levels on serum level at the 5th h after Carr injection. Western blotting revealed that AA decreased Carr-induced inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and nuclear factor-κB (NF-κB) expressions at the 5th h in the edema paw. An intraperitoneal (i.p.) injection treatment with AA also diminished neutrophil infiltration into sites of inflammation as did indomethacin (Indo). The anti-inflammatory mechanisms of AA might be related to the decrease in the level of MDA, iNOS, COX-2, and NF-κB in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver.
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机译：评估了药用植物积雪草中的五环三萜类化合物亚洲酸（AA）的抗伤害感受和抗炎作用。用AA处理雄性ICR小鼠在后期显着抑制了乙酸诱导的扭体反应和福尔马林诱导的疼痛。在抗炎试验中，AA降低了λ-角叉菜胶（Carr）施用后第4和第5小时的爪水肿，并增加了过氧化氢酶（CAT），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。肝组织。在注射Carr后第5小时，AA降低了血清中的一氧化氮（NO），肿瘤坏死因子-α（TNF-α）和白介素1β（IL-1β）水平。 Western blotting结果显示，AA在第5小时的水肿爪中降低了Carr诱导的一氧化氮合酶（iNOS），环氧合酶（COX-2）和核因子-κB（NF-κB）的表达。与消炎痛（Indo）一样，腹膜内（ip。）注射治疗也减少了中性粒细胞浸润到炎症部位。 AA的抗炎机制可能与增加CAT，SOD的活性，降低水肿爪中MDA，iNOS，COX-2和NF- κ B的水平有关，和GPx在肝脏中。
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